Hydrolysis Of Sucrose


Chemical Kinetics involves the determination of velocity or rate of a chemical reaction. The rate reaction depends on the nature of the reactants , the concentration of the reactants, the temperature, and the presence of a catalyst. The concentration of species on the reaction rate are summarized by an equation called the Rate Law for the reaction. Effect of temperature on the reaction rate leads to the energy required for a reaction. Also to determine the rate law, it should be done experimentally. The hydrolysis involves the reaction of sucrose with water in the presence of H+ to form glucose and fructose. Using a spectrophotometer, the concentrations of fructose and glucose that form are measured by reacting them with 3,5-dinitrosalicylate reagent, where they form a colored product that absorbs at a certain wavelength. The absorbance of the solution is then measured and the concentration of fructose and glucose is determined from Beer’s Law. Therefore, the objective of this experiment is to determine the rate of acid-catalyzed hydrolysis of sucrose.

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Before beginning the experiment, safety precautions were observed. We started the experiment by calculating the number of grams of sucrose to prepare 100ml of 0.01M sucrose solution. Our calculations stated that we needed .342g of sucrose. We weighed .345g of sucrose and put it in a 100mL flask half filled with distilled water. We put the sucrose in the flask and made sure that it’s dissolved , and filled the flask with more distilled water until it reached the line indicating it reached 100ml.. We put a stopper on the flask and mixed the solution thoroughly to ensure a solution of uniform concentration. We labeled nine large test tubes 1 to 9. Then we pipet 2.50mL of 3M NaOH into each of the nine test tubes, followed by measuring 30.0mL of 3M HCl in the 50mL graduated cylinder. As soon as Sucrose and HCl solutions were.